Identification of a novel allele HLA-B*15:129 by polymerase chain reaction with allele group-specific primers.
- Author:
Zhe-dong HAN
1
;
Wei WANG
;
Wei ZHANG
;
Jun-jun HE
;
Nan-ying CHEN
;
Fa-ming ZHU
;
Hang-jun LV
;
Li-xing YAN
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Base Sequence; DNA Primers; Exons; HLA-B Antigens; genetics; Humans; Male; Molecular Sequence Data; Molecular Typing; Polymerase Chain Reaction
- From: Chinese Journal of Medical Genetics 2011;28(3):293-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the sequence of the exons 2-4 of human leukocyte antigen (HLA) novel allele HLA-B*15:129.
METHODSDNA of the proband was extracted from whole blood by commercial DNA extraction kit. The amplification for HLA-B exons 2-4 was performed separately by polymerase chain reaction (PCR) with allele group specific primers. The PCR products were digested with enzymes and then directly sequenced for exons 2-4 of HLA-B locus in both directions.
RESULTSSequencing results showed the HLA-B alleles of the proband included B*07:02 and a novel allele. The sequence of the novel allele has been submitted to GenBank (accession no. EF473219) and the allele has been officially named B*15:129 by the WHO Nomenclature Committee. Comparing with the HLA-B*15:01:01:01, the sequence of exons 2-4 of HLA-B*15:129 showed three nucleotide difference in exon 3 at positions 362 and 363 from GG to AT and positions 369 from C to T, which resulted in an amino acid change from Arg to Asn at codon 97.
CONCLUSIONA novel HLA-B allele was identified and has been officially named B15:129 by the WHO Nomenclature Committee.
