Recombination between human leukocyte antigen -A and -C loci within two Chinese Han families.
- Author:
Wei WANG
1
;
Zhe-dong HAN
;
Nan-ying CHEN
;
Jun-jun HE
;
Wei ZHANG
;
Fa-ming ZHU
;
Hang-jun LV
;
Li-xing YAN
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; ethnology; genetics; China; ethnology; Ethnic Groups; genetics; Female; Genetic Loci; genetics; HLA-A Antigens; genetics; HLA-C Antigens; genetics; Haplotypes; genetics; Humans; Male; Pedigree; Recombination, Genetic; genetics
- From: Chinese Journal of Medical Genetics 2011;28(4):417-420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the recombination events between human leukocyte antigen (HLA) loci within two families.
METHODSIdentification of HLA-A, -C, -B, -DRB1 and -DQB1 loci was firstly carried out using polymerase chain reaction-sequence specific oligonucleotide. Then HLA high resolution typing was performed using polymerase chain reaction sequencing-based typing. The recombination between HLA loci was identified by family genetic analysis. The parentage possibility was analyzed by short tandom repeat technique.
RESULTSRecombination between the HLA-A and C loci was identified within two families. One individual inherited a paternal haplotype that was the result of a recombination event between the father's HLA-A and -C loci on his chromosomes. The other individual inherited a maternal haplotype that was the result of a recombination event between the mother's HLA-A and -C loci. The high parentage possibilities were obtained in the family members.
CONCLUSIONThe recombination events of HLA-A and -C have been found in two Chinese families, which may help further study on the mechanism of HLA recombination.
