Association of single nucleotide polymorphism of reduced folate carrier gene with susceptibility to acute leukemia.
- Author:
Wei ZHAO
1
;
Li-jie YUE
;
Xiao-wen CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adolescent; Base Sequence; Child; Child, Preschool; Female; Gene Frequency; Genetic Predisposition to Disease; genetics; Humans; Infant; Infant, Newborn; Leukemia; genetics; Male; Polymorphism, Single Nucleotide; genetics; Reduced Folate Carrier Protein; genetics; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2011;28(4):446-449
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the allele and genotype frequencies of reduced folate carrier gene (RFC) 80G/A polymorphism in Chinese patients with acute leukemia (AL) and healthy control children, and to provide clue for association between the single nucleotide polymorphism (SNP) of RFC and the occurrence of AL.
METHODSBone marrow samples from 98 childhood patients with AL and peripheral blood samples from 135 healthy children were obtained to prepare complementary DNAs (cDNAs). The cDNAs were analyzed for the polymorphisms in RFC 80G/A by reverse transcriptase-polymerase chain reaction-denaturing gradient gel electrophoresis (RT-PCR-DGGE) and direct sequencing.
RESULTSThe A allele frequencies of the AL patients and control children were 0.515 and 0.415, respectively (P< 0.05). Chi-square test confirmed a statistical significance of the association between RFC80 G/A and AL.
CONCLUSIONRFC 80AA or GA genotype may contribute to increasing the susceptibility to AL.
