Effect of magnesium lithospermate B on endothelial cells in human aorta after free radical injury.
- Author:
Zhi-xin ZHANG
1
;
Fu-yun GAO
;
Ke-ming LI
Author Information
- Publication Type:Journal Article
- MeSH: Aorta; cytology; Cell Division; Cells, Cultured; Drugs, Chinese Herbal; pharmacology; Endothelial Cells; pathology; ultrastructure; Free Radical Scavengers; pharmacology; Humans; Oxidative Stress; drug effects; Reactive Oxygen Species; metabolism; Salvia miltiorrhiza; chemistry
- From: Chinese Journal of Integrated Traditional and Western Medicine 2004;24(6):521-524
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cardiovascular effects of magnesium lithospermate B (MLB), a water soluble extract from red sage root, on human aortic endothelial cells (HAECs) proliferation and in treating free radical injured endothelial cells, so as to further understand the cardiovascular pharmacological mechanism of MLB.
METHODSHAECs of 3-6 passages were used in the experiment. MLB of different concentrations was used to treat the cells, and cell proliferation was observed using morphological and MTT method. The free radical injured model cell was made by glucose-glucose oxidase method for observing the effect of MLB on its microstructure by transmission electron microscopy.
RESULTSMLB in concentration of 1.0 mg/ml and 0.5 mg/ml had significant cytotoxicity (P<0.01), when its concentration was lower than 0.2 mg/ml, the drug showed no adverse reaction on cell proliferation. MLB showed significantly cell protective effect against free radical injury, the effect in protecting ultrastructure of cells, such as mitochondria, could be demonstrated in transmission electron microscopy.
CONCLUSIONMLB showed a low cytotoxicity on HAECs, it could obviously protect cells from free radical injury.
