Effects of ecdysterone on the expression of NF-kappaB p65 in H2O2 induced oxidative damage of human lens epithelial cells.
- Author:
Chun-Yan FENG
1
;
Xiu-Rong HUANG
;
Ming-Xin QI
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Ecdysterone; pharmacology; Epithelial Cells; drug effects; metabolism; Humans; Hydrogen Peroxide; adverse effects; Lens, Crystalline; cytology; Oxidative Stress; drug effects; Transcription Factor RelA; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2012;32(1):76-79
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of ecdysterone (ECR) on the expression of nuclear factor (NF)-kappaB in H2O2 induced oxidative damage of human lens epithelial cells (HLECs).
METHODSThe cultured HLECs were divided into 5 groups, i.e., the control group, the H2O2 group, the beta-estradiol (E2) group, the ECR group, and the pyrrolidine dithiocarbamate group (PDTC) group. The expression rate of NF-kappaB p65 in the HLECs were detected by flow cytometer (FCM).
RESULTSThe expression of NF-kappaB p65 occurred in normal HLECs (9. 53%). The expression rate of NF-kappaB p65 in the H2O2 group obviously increased (39.87%, P < 0.01). The expression rate of NF-kappaB p65 in the PDTC group obviously decreased (5.90%, P < 0.01). The expression rates of NF-kappaB p65 in the ECR group (13.99%) and the E2 group (25.18%) ranged between the control group and the H2O2 group, but still lower than that of the H2O2 group (P < 0.01).
CONCLUSIONSThe activation of NF-kappaB in the HLECs could be induced by H2O2 ECR with the estrogenic activity could effectively inhibit the activation of NF-kappaB.
