Isolation And Development Of Quantification Method For Cyanidin-3-glucoside And Cyanidin-3-rutinoside In Mulberry Fruit By High-performance Countercurrent Chromatography And High-performance Liquid Chromatography.
- Author:
Soo Jung CHOI
1
;
Heejin JEON
;
Chang Uk LEE
;
Shin Hee YOON
;
Soo Kyung BAE
;
Young Won CHIN
;
Kee Dong YOON
Author Information
1. College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon 420-743, Korea. kdyoon@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Cyanidin-3-glucoside;
Cyanidin-3-rutinoside;
Mulberry fruit;
High-performance countercurrent chromatography;
HPLC-PDA method
- MeSH:
Calibration;
Chromatography, High Pressure Liquid;
Chromatography, Liquid*;
Countercurrent Distribution*;
Fruit*;
Korea;
Morus*;
Water
- From:Natural Product Sciences
2015;21(1):20-24
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cyanidin-3-glucoside (C3G) and cyanidin-3-rutinoside (C3R) were isolated by high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/water/trifluoroacetic acid (1 : 3 : 1 : 5 : 0.01, v/v) to give pure C3G (34.1 mg) and C3R (14.3 mg) from 1.5 g crude mulberry fruit extract. Using the pure C3G and C3R, a reliable high-performance liquid chromatography (HPLC) method was developed and validated to determine the C3G and C3R contents in mulberry fruit. C3G and C3R were separated simultaneously using an Eclipse XDB-C18 column (4.6 x 250 mm I.D., 5 microm) coupled with a photodiode array detector (PDA). The gradient elution of the mobile phase consisting of acetonitrile (0.5% formic acid) and water (0.5% formic acid) was applied (1.0 mL/min), and the detection wavelength was 520 nm. The calibration curves of C3G and C3R showed good linearity (both with r2 = 0.9996) in the concentration range 15.625 - 500 microg/mL, and the relative standard deviations (RSD%) of intra- and interday variability were in the ranges 2.1 - 8.2% and 4.1 - 17.1%, respectively. The accuracies were ranged 96.5 - 102.6% for C3G and C3R, respectively. The developed HPLC method was used to determine the contents of C3G and C3R in newly harvested mulberry from eight different provinces of Korea.
