Dynamic observation of intestinal epithelial stem cells of small intestine during the injured-repaired progress induced by 5-FU.
- Author:
Cheng-tang WU
1
;
Yu-qi LUO
;
Yin WEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Epithelial Cells; cytology; drug effects; Female; Fluorouracil; adverse effects; Intestinal Mucosa; cytology; drug effects; pathology; Intestine, Small; cytology; drug effects; pathology; Intestines; pathology; Male; Mice; Mice, Inbred C57BL; Stem Cells; drug effects
- From: Chinese Journal of Gastrointestinal Surgery 2008;11(6):572-575
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the dynamic changes of intestinal epithelial stem cells during the injured-repaired progress induced by 5-FU.
METHODSFifty adult C57BL/6J mice were enrolled in this study, 40 of them were intraperitoneally injected with 5-FU (30 mg per kg of body weigh) for five days, and 10 of them intraperitoneally injected with PBS as control. At day 1, 3, 5, 7 after treatment, the mice were killed and middle intestine was taken. Pathology was examined by HE staining. Musashi-1 (msi-1) expression was detected by immunohistochemical technique. The percentage of Rho low staining cells was detected by flow cytometry.
RESULTSAfter treatment with 5-FU, the intestinal mucosa was damaged. The Rho low staining cells were increasing, and at day 1 after treatment, the percentage of Rho low staining cells reached the highest level (P<0.01). The number of cells expressing msi-1 did not change significantly (P>0.05), but the percentage of positive msi-1 cells increased significantly (P<0.01). There was positive correlation between the percentage of Rhodamine 123 low staining cells and positive msi-1 cells in each group (r=0.867, P<0.01).
CONCLUSIONSThe Rho low staining cells may contain rich intestinal epithelial stem cells. The intestinal epithelial stem cells expressing msi-1 can regenerate the damage of intestinal mucosa induced by 5-FU.
