Effects of Cynaroside, Cynarin and Linarin on Secretion, Production and Gene Expression of Airway MUC5AC Mucin in NCI-H292 Cells.
- Author:
Yong Pill YOON
1
;
Hyun Jae LEE
;
Young Ho KIM
;
Bui Thi Thuy LUYEN
;
Jang Hee HONG
;
Choong Jae LEE
Author Information
1. Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon, Korea. LCJ123@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Airway;
MUC5AC;
Linarin;
Cynarin;
Cynaroside
- MeSH:
Chrysanthemum;
Enzyme-Linked Immunosorbent Assay;
Epidermal Growth Factor;
Epithelial Cells;
Gene Expression*;
Mucins*;
Necrosis;
Tumor Necrosis Factor-alpha
- From:Natural Product Sciences
2015;21(1):59-65
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we investigated whether cynaroside, cynarin and linarin derived from Chrysanthemum indicum L. affect the secretion, production and gene expression of MUC5AC mucin in airway epithelial cells. Confluent NCI-H292 cells were pretreated with cynaroside, cynarin or linarin for 30 min and then stimulated with PMA (phorbol 12-myristate 13-acetate) for 24 h. The MUC5AC mucin gene expression, mucin protein production and secretion were measured by RT-PCR and ELISA, respectively. Effect of linarin on EGF (epidermal growth factor) - or TNF-alpha (tumor necrosis factor-alpha)-induced MUC5AC mucin gene expression and mucin protein production was also examined. The results were as follows: (1) Cynaroside and cynarin did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, linarin decreased MUC5AC mucin secretion; (2) Cynaroside did not affect PMA-induced MUC5AC mucin production and gene expresion from NCI-H292 cells. However, cynarin and linarin inhibited the production and gene expression of MUC5AC mucin; (3) Linarin also inhibited the production and gene expression of MUC5AC mucin induced by EGF- or TNF-alpha from NCI-H292 cells. These results suggest that linarin can regulate the gene expression, production and secretion of mucin, by directly acting on airway epithelial cells.
