Regulation of immune suppressive cytokines by TLR4 activation in colon cancer cells.
- Author:
Hong-ming QI
1
;
Tie-ming ZHU
;
Jian WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chemokine CCL20; metabolism; Colonic Neoplasms; immunology; metabolism; Cytokines; immunology; HT29 Cells; Humans; Interleukin-6; metabolism; NF-kappa B; metabolism; RNA, Messenger; genetics; Signal Transduction; Toll-Like Receptor 4; immunology; metabolism; Transforming Growth Factor beta; metabolism; Up-Regulation
- From: Chinese Journal of Gastrointestinal Surgery 2009;12(4):413-415
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulation of Toll-like receptors (TLRs) on immune suppressive cytokines in situ colon cancer cells.
METHODSThe mRNA and protein expression spectrum of TLRs in HT-29 cells were determined by RT-PCR and Western blot respectively. The cytokines and chemokines levels of supernant of HT-29 stimulated by lipoplysaccharide(LPS) were detected with ELISA.
RESULTSTLR1-9 were expressed in HT-29 cells on mRNA level. After LPS stimulation, TLR4 mRNA and protein expressions were up-regulated in HT-29 cells, and TGF-beta, VEGF, IL-8, CCL20 and IL-6 levels increased significantly(all P<0.01). Except IL-6, up-regulation of the other cytokines was not suppressed by NF-kappa B inhibitor.
CONCLUSIONTLRs expressed on colon cancer cells may elevate the immune suppressive cytokines and chemokines, which promote the immune escape of cancer cells.
