Adenovirus mediated N19RhoA gene transfer attenuates neointimal formation in rats after carotid artery balloon injury.
- Author:
Yu-feng CHU
1
;
Wen-dong CHEN
;
Jin-jiao JIANG
;
Mei MENG
;
Juan ZENG
;
Chun-ting WANG
;
Ping-jin GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Animals; Carotid Arteries; metabolism; Carotid Artery Injuries; metabolism; pathology; Genetic Vectors; Male; Muscle, Smooth, Vascular; metabolism; Neointima; Rats; Rats, Sprague-Dawley; Transfection; rhoA GTP-Binding Protein; genetics
- From: Chinese Journal of Cardiology 2012;40(7):601-606
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of small G-protein RhoA in neointimal formation following rat carotid artery balloon injury and related mechanisms.
METHODSMale 3-4-month-old Sprague-Dawley rats were used in the present study (10 rats per group). Group A: control; Group B: carotid artery balloon injury; Group C: injury + Ad-CMV-eGFP + Pluronic F-127; Group D: injury + Ad-CMV-N19RhoA-eGFP + Pluronic F-127; Group E: non injury + Ad-CMV-eGFP + Pluronic F-127. Perivascular gene transfer of an adenovirus co-expressing N19RhoA was performed to rat carotid artery following balloon injury and the effect on neointimal formation and the expressions of PCNA and α-SM-actin examined. Rats were killed after 14 days.
RESULTSThe protein expression of RhoA in group B was significantly higher than in group A (P = 0.001), and the positive cells rate of PCNA and α-SM-actin which were assessed by immunohistochemistry in group C (45.2% and 75.6%) was significantly higher than in group D (28.4% and 51.9%, all P < 0.01). The area of neointima was significantly smaller [(0.14 ± 0.08) mm(2) vs. (0.23 ± 0.10) mm(2), P < 0.01], the luminal area was significantly larger [(0.47 ± 0.11) mm(2) vs. (0.31 ± 0.06) mm(2), P < 0.01] in group D than in group C.
CONCLUSIONGene transfer of N19RhoA attenuates neointimal formation after balloon injury in rat carotid arteries possibly related to the modulating capacities of small G-protein RhoA on the proliferation, phenotypic differentiation and migration of vascular adventitial fibroblasts.
