Adenovirus mediated N19RhoA gene transfer attenuates neointimal formation in rats after carotid artery balloon injury
10.3760/cma.j.issn.0253-3758.2012.07.013
- VernacularTitle:腺病毒介导N19RhoA基因转染抑制大鼠颈动脉球囊损伤后新生内膜形成
- Author:
Yu-Feng CHU
1
;
Wen-Dong CHEN
;
Jin-Jiao JIANG
;
Mei MENG
;
Juan ZENG
;
Chun-Ting WANG
;
Ping-Jin GAO
Author Information
1. 山东大学附属省立医院
- Keywords:
GTP-binding proteins;
Tunica intima;
Transfection
- From:
Chinese Journal of Cardiology
2012;40(7):601-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of small G-protein RhoA in neointimal formation following rat carotid artery balloon injury and related mechanisms.Methods Male 3-4-month-old SpragueDawley rats were used in the present study ( 10 rats per group).Group A:control; Group B:carotid artery balloon injury; Group C:injury + Ad-CMV-eGFP + Pluronic F-127; Group D:injury + Ad-CMV-N19RhoA -eGFP + Pluronic F-127 ; Group E:non injury + Ad-CMV-eGFP + Pluronic F-127.Perivascular gene transfer of an adenovirus co-expressing N19RhoA was performed to rat carotid artery following balloon injury and the effect on neointimal formation and the expressions of PCNA and α-SM-actin examined.Rats were killed after 14 days.Results The protein expression of RhoA in group B was significantly higher than in group A ( P =0.001 ),and the positive cells rate of PCNA and α-SM-actin which were assessed by immunohistochemistry in group C (45.2％ and 75.6％ ) was significantly higher than in group D ( 28.4％and 51.9％,all P ＜ 0.01 ).The area of neointima was significantly smaller [ (0.14 ± 0.08) mm2 vs.(0.23 ± 0.10) mm2,P ＜ 0.01 ],the luminal area was significantly larger [ (0.47 ± 0.11 ) mm2 vs.(0.31 ±0.06) mm2,P ＜ 0.01 ] in group D than in group C.Conclusion Gene transfer of N19RhoA attenuates neointimal formation after balloon injury in rat carotid arteries possibly related to the modulating capacities of small G-protein RhoA on the proliferation,phenotypic differentiation and migration of vascular adventitial fibroblasts.
