Nuclear factor κB and IKB expression and calcium deposition of atherosclerotic plaques in apolipoprotein E and low density lipoprotein receptor knockout mice
10.3760/cma.j.issn.0253-3758.2012.08.013
- VernacularTitle:载脂蛋白E基因敲除小鼠和低密度脂蛋白受体基因敲除小鼠动脉粥样硬化斑块内核因子κB及其抑制蛋白的表达与钙沉积
- Author:
Feng-Zhen YANG
1
;
Jun ZHOU
;
Wen-Wen LI
;
Fang WANG
;
Pu-Yuan WEN
;
Li ZHOU
;
Jian-Gang WANG
;
Xing-Xing ZHENG
Author Information
1. 中南大学湘雅医学院
- Keywords:
Atherosclerosis;
NF-kappa B
- From:
Chinese Journal of Cardiology
2012;40(8):684-689
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the histopathological features,nuclear factor-κB (NFκB) and IKB expressions as well as calcium deposition of atherosclerosis plaques (AS) in apolipoprotein E (ApoE) and low density lipoprotein receptor (LDLR) knockout mice (ApoE-/-, LDLR-/- fed high-fat diet.Methods Eight C57BL/6J mice fed with normal diet were used as control,32 ApoE-/- mice and LDLR -/- mice were divided into normal diet and high-fat diet groups ( n =8 each).After 4 months,aorta was collected for morphologic ( HE,Oil Red O,Von Kossa ) and immunohistochemistry ( nuclear factor-κB,IKB,macrophage surface molecule-3, α-smooth activor protein ) analysis.Results Degree of AS in ApoE -/ - and LDLR-/- mice fed with high-fat diet were significantly severer than those fed with normal diet and AS was more significant in ApoE-/- mice than in LDLR -/- mice.NFκB and IKB expressions in high-fat diet group were significantly higher than the normal diet group ( P ＜ 0.05 ). Double-labeling of NFκB revealed dominant expression in smooth muscle cells.Calcium deposition was significantly more in ApoE-/- mice fed with high-fat diet than mice fed with normal diet (P ＜0.05) and was similar in LDLR-/- mice fed with high and normal diet (P ＞ 0.05 ).Conclusion High-fat diet contributes to the formation of AS plagues in ApoE -/- and LDLR -/- mice joined by upregulated NFκB and IKB expressions and calcium deposition.
