The effect of CD137-CD137 ligand interaction on the expression of nuclear factor of activated T cells c1 in apolipoprotein E-deficient mice atherosclerotic plaque model.
- Author:
Hai-bing YANG
1
;
Jin-chuan YAN
;
Hong-ling SU
;
Wei YUAN
;
Liang-jie XU
Author Information
- Publication Type:Journal Article
- MeSH: 4-1BB Ligand; metabolism; Animals; Apolipoproteins E; genetics; Disease Models, Animal; Mice; Mice, Knockout; NFATC Transcription Factors; metabolism; Plaque, Atherosclerotic; metabolism; RNA, Messenger; genetics; Tumor Necrosis Factor Receptor Superfamily, Member 9; metabolism
- From: Chinese Journal of Cardiology 2012;40(9):775-779
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of CD137-CD137L interaction on the nuclear factor of activated T cells c1 (NFATc1) in apolipoprotein E knockout (ApoE(-/-)) mice.
METHODSAtherosclerotic plaque model was produced by rapid perivascular carotid collar placement in ApoE(-/-) mice. In vivo, the expression of NFATc1 in mice plaque and lymphocytes was detected by immunohistochemical and flow cytometry, respectively. In vitro, the NFATc1 mRNA and protein expressions in cultured lymphocytes of ApoE(-/-) mice were measured by RT-PCR and flow cytometry, respectively.
RESULTSIn vivo, after stimulating CD137-CD137L signal pathway, the expression of NFATc1 was significantly upregulated in the atherosclerotic plaques and lymphocytes. In vitro, the mRNA and protein expressions of NFATc1 in cultured leukocytes of ApoE(-/-) mice were also significantly increased, the maximal effect appeared post 20 µg/ml anti-CD137 mAb-stimulation and reached maximum at 24 h at any concentrations. Anti-CD137L mAb significantly downregulated the mRNA and protein expressions of NFATc1 in lymphocytes of ApoE(-/-) mice, maximal effect appeared at 20 µg/ml anti-CD137L mAb and reached minimum at 24 h.
CONCLUSIONCD137-CD137L interactions can modulate the expression of NFATc1 in this ApoE(-/-) mice atherosclerotic plaque model.
