The effect of CD137-CD137 ligand interaction on the expression of nuclear factor of activated T cells c1 in apolipoprotein E-deficient mice atherosclerotic plaque model
10.3760/cma.j.issn.0253-3758.2012.09.012
- VernacularTitle:干预CD137-CD137配体轴对载脂蛋白E基因敲除小鼠活化T细胞核因子c1表达的影响
- Author:
Hai-Bing YANG
1
;
Jin-Chuan YAN
;
Hong-Ling SU
;
Wei YUAN
;
Liang-Jie XU
Author Information
1. 江苏大学医学院附属医院
- Keywords:
Atherosclerosis;
Antigens,CD137;
NFATC transcription factors
- From:
Chinese Journal of Cardiology
2012;40(9):775-779
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of CD137-CD137L interaction on the nuclear factor of activated T cells c1 (NFATc1) in apolipoprotein E knockout (ApoE-/-) mice.Methods Atherosclerotic plaque model was produced by rapid perivascular carotid collar placement in ApoE-/- mice.In vivo,the expression of NFATc1 in mice plaque and lymphocytes was detected by immunohistochemical and flow cytometry, respectively.In vitro, the NFATc1 mRNA and protein expressions in cultured lymphocytes of ApoE -/- mice were measured by RT-PCR and flow cytometry,respectively.Results In vivo,after stimulating CD137-CD137L signal pathway, the expression of NFATc1 was significantly upregulated in the atherosclerotic plaques and lymphocytes.In vitro,the mRNA and protein expressions of NFATc1 in cultured leukocytes of ApoE-/- mice were also significantly increased,the maximal effect appeared post 20 μg/ml anti-CD137 mAb- stimulation and reached maximum at 24 h at any concentrations.Anti-CD137L mAb significantly downregulated the mRNA and protein expressions of NFATcl in lymphocytes of ApoE -/- mice,maximal effect appeared at 20 μg/ml anti-CD137L mAb and reached minimum at 24 h.Conclusion CD137-CD137L interactions can modulate the expression of NFATc1 in this ApoE -/- mice atherosclerotic plaque model.
