Effect of hepatocyte growth factor and transforming growth factor-β1 on atrial fibroblasts fibrosis
10.3760/cma.j.issn.0253-3758.2012.10.007
- VernacularTitle:肝细胞生长因子和转化生长因子β1对人心房肌成纤维细胞纤维化的影响
- Author:
Jian-Cheng ZHANG
1
;
Jian-Quan CHEN
;
Chun-Xuan XU
;
Lin CHEN
;
Ya-Zhou LIN
;
Guo-Sheng WU
Author Information
1. 福建省立医院心血管内科
- Keywords:
Atrial fibrillation;
Fibroblasts;
Hepatocyte growth factor;
Transforming growth factor bata1
- From:
Chinese Journal of Cardiology
2012;40(10):834-839
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hepatocyte growth factor (HGF) and transforming growth factor-β1 (TGFβ1) on the expression of α-smooth muscle actin (α-SMA) and collagen Ⅰ in human atrial fibroblast in vitro,and to explore the possible molecular mechanism of atrial fibrosis in patients with atrial fibrillation (AF).Methods Human atrial fibroblast,isolated from aseptic right atrial appendage tissues of 10 sinus rhythm (SR) and 10 chronic atrial fibrillation (CAF) patients,were cultured with HGF and TGFβ1.mRNA expressions of collagen Ⅰ and α-SMA were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR),the protein expression of α-SMA was determined by immunofluorescence and Western blot.Results (1) Compared with SR group,left atrium was significantly dilated in CAF group(t =2.692,P ＜0.05),the mRNA expression of collagen Ⅰ and α-SMA of atrial fibroblasts were significantly upregulated (all P ＜ 0.01),mRNA expression of collagen Ⅰ was positively correlated with left atrial dimension (LAD) (r =0.836,P =0.014),AF duration (r =0.739,P =0.045) and α-SMA mRNA level (r =0.886,P =0.012).(2) Compared with SR group,the expression of α-SMA protein in CAF atrial fibroblasts were significantly increased (P ＜ 0.01).(3) TGFβ1 further stimulated while HGF significantly attenuated the expression of collagen Ⅰ and α-SMA in CAF atrial fibroblasts (all P ＜0.01).Conclusions Increasing expression of collagen Ⅰ and α-SMA in human atrial fibroblasts might promote atria remodeling leading to the development and sustaining of AF.HGF is involved in the negative regulation on the expression of α-SMA and collagen Ⅰ.
