Effect of ginsenoside on fine particulate matter induced oxidative injury in human endothelial cells
10.3760/cma.j.issn.0253-3758.2012.10.013
- VernacularTitle:细颗粒物致人脐静脉内皮细胞氧化损伤及人参皂甙Rg1对其干预效果的实验研究
- Author:
Gui-Jin MA
1
;
Ji-Yuan L(U)
;
Ming-Sheng ZHANG
;
Cai-Ping LI
;
Gang QIN
Author Information
1. 山西医科大学第一附属医院
- Keywords:
GINSENOSIDE;
Oxidativc stress;
Umbilical veins;
Endothelium,vascular;
PM2.5
- From:
Chinese Journal of Cardiology
2012;40(10):864-868
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of fine particulate matter (PM2.5) induced endothelial injury and the efficacy and mechanism of ginsenoside Rg1 on the inhibition of endothelium injuries in human endothelial cells exposured to PM2.5.Methods Human umbilical vein endothelial cells (HUVECs) were stimulated with various concentrations PM2.5 (0.1,0.2,0.4,0.8 mg/ml) and PM2.5 at concentration 0.8 mg/ml induced significant endothelial injury and was chosen for the main study in the presence or absence of Rg1 (0.04 mg/ml).After 24 h treatment,cell growth A value was detected through MTT,intracellular reactive oxygen species (ROS) level through fluorescence labeling probe method and HO-1,Nrf2 mRNA expression was detected by RT-PCR.Results The cell A value was significantly lower while the ROS fluorescence gray value and the average optical density ratio of HO-1 were significantly higher in PM2.5 group than in the control group (all P ＜ 0.05).The average optical density ratio of Nrf2 was similar between PM2.5 group and control group (P ＞ 0.05).The A value and the average optical density ratio of HO-1 were significantly higher while the ROS fluorescence gray value was significantly lower in co-treated PM2.5 (0.8mg/ml) + Rgl (0.04 mg/ml) group than in the PM2.5 (0.8 mg/ml) group (all P ＜ 0.05).Conclusion PM2.5 could induce human endothelial cells injury by increasing oxidative stress which could be attenuated by ginsenoside Rg1.
