Identification of genes regulated by HBV preS1-transactivated protein 2 binding protein 1.
- VernacularTitle:乙型肝炎病毒前S1蛋白反式激活蛋白2结合蛋白1基因的克隆及其反式调节基因的筛选
- Author:
Huan-hu ZHANG
1
;
Dan-qiong WANG
;
Jun CHENG
;
Jie ZHAO
;
Xin-kai ZHAO
;
Hong GAO
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Carrier Proteins; Cloning, Molecular; Gene Library; Genetic Vectors; Hep G2 Cells; Hepatitis B Surface Antigens; genetics; Hepatitis B virus; Humans; Molecular Sequence Data; Protein Precursors; genetics; Trans-Activators; genetics
- From: Chinese Journal of Hepatology 2010;18(4):267-270
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify genes regulated by HBV preS1-transactivated protein 2 binding protein 1 (PS1TP2BP1).
METHODSPS1TP2BP1 gene was amplified by polymerase chain reaction (PCR) technique and cloned into the eukaryotic expression vector pcDNA 3.1/my-c-His A. The mRNAs isolated from HepG2 cells transfected recombinant eukaryotic expression vector pcDNA 3.1/myc-HisA-PS1TP2BP1 and pcDNA 3.1/myc-HisA empty vector were used to construct subtractive library. The differentially expressed genes were identified and analyzed.
RESULTS35 differentially expressed clones were obtained. Colony PCR identified 15 clones with 200-1000 bp inserts. Sequence analysis identified 15 differentially expressed genes.
CONCLUSIONThis study provides data for further characterize the function of PS1TP2BP1.
