Effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase on hepatocellular carcinoma in mice.
- Author:
Shi-ji ZHOU
1
;
Chan-an LIU
;
Jian-ping GONG
;
Zuo-jin LIU
;
Yong TANG
;
Sheng-wei LI
;
Yue XU
;
Zhi-guo AI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carcinoma, Hepatocellular; therapy; Cell Line, Tumor; Genes, Transgenic, Suicide; Genetic Therapy; Liver Neoplasms; therapy; Male; Mice; Mice, Inbred Strains; Microbubbles; Simplexvirus; genetics; metabolism; Thymidine Kinase; genetics; Treatment Outcome; Ultrasonics
- From: Chinese Journal of Hepatology 2010;18(4):276-279
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of ultrasound microbubble carrying herpes simplex virus thymidine kinase hepatocellular carcinoma in mice.
METHODSKunming mice were inoculated subcutaneously with H22 tumor cells. 40 male mice bearing subcutaneous hepatoma were randomized into 4 groups: PBS (group A), HSV1-TK (group B), HSV1-TK (group C), and microbubble carrying HSV1-TK (group D) were injected into the tail vein every 3 days. Mice in group C and D were exposed to ultrasound. The expression of TK protein was detected by western blot. Ganciclovir (GCV) was intraperitoneally injected at a dose of 100 mg x kg (-1) x d(-1) in group B, group C and group D. The tumor size was measured every 2 days.
RESULTSTK gene could be injected precisely into hepatocellular carcinoma with ultrasound monitor, and the expression of TK protein was found in all 4 groups. Expression in group D was higher than others (P < 0.05). The rate of tumor growth inhibition were 0 in group A, 3.90%+/-1.80% in group B, 22.70%+/-2.86% in group C, 41.25%+/-3.20% in group D (group B vs group C, P < 0.05; group D vs group C, P < 0.05; group D vs group B, P < 0.05).
CONCLUSIONUltrasound microbubble not only improve target gene therapy, but also enhance transfection efficiency.
