Effects of pravastatin on the proliferation and invasion of human hepatocarcinoma HepG2 cell line.
- VernacularTitle:普伐他汀对HepG2细胞增殖及侵袭能力的影响
- Author:
Wen-jie ZHANG
1
;
Shao-hua YANG
;
Shu-ju TIAN
;
Zhao-xia LI
;
Yan-xuan GONG
;
Yan QU
;
Wei-wu MAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Movement; drug effects; Cell Proliferation; drug effects; Hep G2 Cells; Humans; Matrix Metalloproteinase 2; metabolism; Neoplasm Invasiveness; Pravastatin; pharmacology
- From: Chinese Journal of Hepatology 2010;18(4):280-283
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of pravastatin on the proliferation and invasion of human hepatocarcinoma HepG2 cell line.
METHODSThe effects of pravastatin on the proliferation, migration and invasion of HepG2 cells was observed by MTT assay, Boyden chamber assay and motility assay. p38 activity was measured, and the expression of p-p38, MKP-1, RhoC and MMP-2 was analyzed by Western blot.
RESULTSPravastatin inhibited the proliferation of HepG2 cells. The intracellular p38 activity and expressions of p-p38, RhoC and MMP-2 were decreased, while MKP-1 expression was elevated in pravastatin treated cells. In addition, pravastatin inhibited the invasion and motility.
CONCLUSIONPravastatin can inhibit the proliferation and invasion of HepG2 cells.
