No correlation between the sensitivity to 5-aza-dC and the global DNA methylation level in hepatocellular carcinoma cells.
- Author:
Xiao-ping LI
1
;
Ai-long HUANG
;
Mei YANG
Author Information
- Publication Type:Journal Article
- MeSH: Azacitidine; analogs & derivatives; pharmacology; Carcinoma, Hepatocellular; metabolism; Caspase 3; metabolism; DNA Methylation; drug effects; DNA Modification Methylases; antagonists & inhibitors; Hep G2 Cells; Humans
- From: Chinese Journal of Hepatology 2010;18(4):284-287
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the sensitivity of different hepatocellular carcinoma (HCC) cell lines (HepG2, QGY7701, HepG2.2.15) and the normal liver cell line L02 to 5-aza-dC, an DNA methyltransferase inhibitor, and to explore the relationship between global DNA methylation level and the sensitivity to 5-aza-dC.
METHODSHepG2, QGY7701, HepG2.2.15 and L02 cells were treated with 5-aza-dC at different concentration, cell proliferation was measured by MTT method, cell apoptosis was detected by measuring caspase 3 activity and cellular DNA fragmentation ELISA.
RESULTSCompared to HepG2 and QGY7701 cells, HepG2.2.15 were less sensitive to the treatment of 5-aza-dC; the normal liver cell line L02 was less sensitive to 5-aza-dC than the HCC cell lines.
CONCLUSIONSThe sensitivity to 5-aza-dC of HCC cell lines and normal liver cells is not correlated with the global DNA methylation level.
