OBJECTIVETo compare two models of nonalcoholic hepatocellular steatosis.

METHODSHL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50% fetal bovine serum to induce fat-overloading. Significant fat accumulation was documented by Oil Red O staining , and intracellular triglyceride levels was detected by triglyceride enzymatic assay.

RESULTSThe results showed that both 0.5 mmol/ml oleate acid and 50% FBS were able to induce nonalcoholic hepatocellular steatosis.

CONCLUSIONA nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.