Effects of insulin and LY294002 inhibitors of PI3K on the regulations and expression of aquaporin 9 in normal liver cells.
- VernacularTitle:胰岛素对人肝细胞L02水通道蛋白9表达的影响及其调控通路
- Author:
Hui LIU
1
;
Zhe-Chuan MEI
;
Xiao XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Aquaporins; metabolism; Cells, Cultured; Chromones; pharmacology; Hepatocytes; drug effects; metabolism; Humans; Insulin; pharmacology; Morpholines; pharmacology; Phosphatidylinositol 3-Kinase; antagonists & inhibitors; metabolism; Protein-Serine-Threonine Kinases; metabolism; Signal Transduction
- From: Chinese Journal of Hepatology 2010;18(6):455-458
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo explore the effects of insulin on the expression and the regulatory pathway of AQP9 in normal human liver cells.
METHODSNormal human liver cells L02 were cultured and treated with PI3K inhibitor LY294002, AKT inhibitor A-443654, MAPK inhibitors SB2030580 and insulin at different concentrations respectively. The AQP9 mRNA and protein expressions were detected with semi-quantitative RT-PCR and Western blot respectively.
RESULTSThe insulin (100 nmol/L approximately 500 nmol/L) treatment decreased the expression of AQP9 in normal human liver cells (P less than 0.05) concentration dependently, and the expression of AQP9 began to reduce from 3 hours of insulin stimulation (P less than 0.05), especially at insulin treatment for 12 hours (P less than 0.05); Incubated with the selective inhibitor of PI3K (LY294002) and AKT (A-443654), the inhibitory effects of insulin on AQP9 expression decreased (P less than 0.05); but it did not change significantly by blocking the MAPK signaling pathway.
CONCLUSIONThe insulin treatment inhibited the expression of AQP9 and the PI3K/akt signal transduction pathway was involved in the mechanism.
