Readspace solid-phase microextraction-gas chromatography for determination of 2,5-hexanedione in urine.
- Author:
Wei ZOU
;
Mengping ZHANG
;
Haiyan WEI
- Publication Type:Journal Article
- MeSH: Chromatography, Gas; Hexanones; urine; Humans; Sensitivity and Specificity; Solid Phase Microextraction
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(4):297-298
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determination of 2,5-hexanedione in urine by headspace solid-phase microextraction-gas chromatography.
METHODSAfter extraction by solid-phase microextraction head, 2,5-hexanedione in urine was determined by gas chromatography and was quantified by external standard method.
RESULTSThe concentration of 2,5-hexanedione in urine showed a linear relationship within the range of 0.1-20.0 µg/ml. The regression equation was y=261.36x-1.903 3, r=0.999 2. The minimum detectable concentration was 0.01 µg/ml. The recovery rate was 92.6%-97.1%, with a relative standard deviation (RSD) of 3.3%-5.8%. The intra-day and inter-day RSDs were 3.8%-6.2% and 4.7%-6.3% respectively.
CONCLUSIONThis determination method has no requirement for organic solvents, features simple and rapid operation, possesses higher detection sensitivity, and applies well to the determination of 2,5-hexanedione in urine.