Effect of METH1 gene transfection on the proliferation of rabbit's ear scar.
- Author:
Bao-Qiang SONG
1
;
Kai-Hua LU
;
Shu-Zhong GUO
;
Yang ZHANG
;
Pai PENG
;
Fu-Cheng MA
;
Hui-Yuan LI
Author Information
- Publication Type:Journal Article
- MeSH: ADAM Proteins; genetics; Angiogenesis Inhibitors; genetics; Animals; Cicatrix, Hypertrophic; genetics; pathology; Disease Models, Animal; Ear; pathology; Ear, External; pathology; Female; Male; Microcirculation; Neovascularization, Pathologic; Rabbits; Transfection; Wound Healing
- From: Chinese Journal of Plastic Surgery 2008;24(2):148-150
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of METH1 gene transfection on fibroblast proliferation and I, III collagen synthesis in rabbit ear scar.
METHODSThe hypertrophic scar model on the rabbit ears was reproduced. 10 days after epithelization, Ad-METH1 was injected into the scar tissue. 30 days later, the effect of METH1 gene transfection on the angiogenesis, fibroblast proliferation and the ratio of collagen I/III in the scar tissue was detected by microcirculation microscope, AgNOR particle count and collagen dyeing.
RESULTS30 days after injection of Ad-METH1, angiogenesis, fibroblast proliferation and the ratio of collagen I/III in the scar tissue were obviously suppressed.
CONCLUSIONEarly application of Ad-METH1 after epithelization can markedly inhibit the formation of the hypertrophic scar.
