Experimental study of electroporation-mediated plasmid gene expression in skin and incisional wound.
- Author:
Zhen GAO
1
;
Nan SONG
;
Xiao-Li WU
;
Yi-Lin CAO
;
Wei LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Electroporation; Gene Expression; Genetic Vectors; Green Fluorescent Proteins; genetics; Plasmids; Rats; Rats, Sprague-Dawley; Skin; injuries; metabolism; Transfection
- From: Chinese Journal of Plastic Surgery 2008;24(5):390-393
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility of electroporation mediated gene transfer in rat incisional wound.
METHODS12 Sprague-Dawley rat's dorsal skins were electroporated (800 voltages in amplitude with 6 square wave pulses, each lasting 20 milliseconds with 200 millisecond interval) after injection of plasmid DNA (1 microg/microl, in 100 microl PBS) containing enhanced green fluorescence protein (EGFP) gene. Electroporated skins were incisionally wounded 24 hours after electroporation. Specimens were harvested at day 2, 4, 6, 14, then EGFP expression in dermis was observed and quantitatively analyzed with integrated optical density (IOD) followed by H&E staining.
RESULTSElectroporation can mediate EGFP expression in epidermis, dermis and panniculus muscle. The expression level in dermis was the highest at day 2 (IOD = 3.50 +/- 1.45) and disappeared at day 14. EGFP expression was not found in dermis if no electroporation applied after plasmid injection (IOD = 0).
CONCLUSIONElectroporation can mediate plasmid gene expression in incisional wound efficiently and widely.
