OBJECTIVETo research adipogenic effect of human adipose tissue derived stem cells (ADSC) obtained from fluid portion of liposuction aspirates in nude mouse.

METHODSADSC were isolated from the fluid portion of human liposuction aspirates and cultured. The cells were incubated in either a adipogenic or an osteogenic medium or a chondrogenic medium for 2-4 weeks to induce adipogenesis or osteogenesis or chondrogenesis, respectively. Adipogenesis was examined in nude mice imbedded subcutaneously with collagen sponge (group 1), or collagen sponge seeded with ADSC (group 2), or collagen sponge seeded with adipogenic differentiated ADSC (group 3). Histologic examination, HE and oil red O staining were used to assess the newly formed tissue.

RESULTSA large number of ADSC were obtained from the fluid portion of human liposuction aspirates. The ADSC were differentiated into adipose tissue, bone and cartilage. 8 weeks after implantation, the collagen sponge was fully absorbed in group 1. Newly formed adipose tissue was observed in groups 2 and 3, but much more in group 3.

CONCLUSIONSThe ADSC from the fluid portion of human liposuction aspirates can be cultured with collagen framework to form adipose tissue in vivo. It indicates that ADSC can be used as seed cells in adipose tissue engineering.