Comparison of the mechanisms of intralesional steroid, interferon or verapamil injection in the treatment of proliferative scars.
- Author:
Shao-jun XU
1
;
Jian-ying TENG
;
Jing XIE
;
Ming-qiang SHEN
;
Dong-ming CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Apoptosis; Child; Cicatrix, Hypertrophic; drug therapy; Female; Humans; Interferon-alpha; therapeutic use; Male; Proliferating Cell Nuclear Antigen; metabolism; Recombinant Proteins; Steroids; therapeutic use; Transforming Growth Factor beta1; metabolism; Verapamil; therapeutic use; Young Adult
- From: Chinese Journal of Plastic Surgery 2009;25(1):37-40
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of intralesional steroid, interferon alpha-2b or verapamil injection on proliferation, apoptosis and TGF-beta1 expression in keloid and hypertrophic scar in vivo.
METHODS6 patients with keloids and 6 patients with hypertrophic scar were treated with intralesional injection of triamcinolone acetonide (40 mg/ml) or IFN alpha-2b (15 x 10(5) U/ml) or verapamil (2.5 mg/ml). Samples were collected on the 7th day after intralesional injection. Samples of untreated keloid and hypertrophic scar and normal skin were used as control. Expression of PCNA and TGF-beta1 was detected in situ by immunohistochemical staining, and apoptosis was detected in situ by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL).
RESULTS1) Triamcinolone acetonide could prohibit proliferative scars through inhibiting cell proliferation and TGF-beta1 expression, as well as inducing apoptosis. 2) IFN alpha-2b could prohibit proliferative scars through inhibiting cell proliferation and TGF-beta1 expression, but not inducing apoptosis; 3) Verapamil could also prohibit proliferative scars through inhibiting proliferation and TGF-beta1 expression in fibroblasts, as well as inducing apoptosis. While the effect of inducing apoptosis was stronger than that of triamcinolone acetonide, the effect of inhibiting TGF-beta1 expression was weaker than those of triamcinolone acetonide and IFN alpha-2b.
CONCLUSIONSAlthough intraleional injection of steroid, interferon alpha-2b or verapamil were all effective in the treatment of keloid and hypertrophic scar, their mechanisms are not similar.