Detection of human cyclin C gene expression in childhood acute lymphocytic leukemia using real-time fluorescence quantitative PCR.
- Author:
Zhao-Xia ZHANG
1
;
Li-Zhi CAO
;
Qiong HUANG
;
Ming-Hua YANG
;
Zhuo WANG
;
Yan YU
Author Information
- Publication Type:Journal Article
- MeSH: Child; Cyclin C; Cyclins; genetics; Female; Fluorescence; Humans; Male; Polymerase Chain Reaction; methods; Precursor Cell Lymphoblastic Leukemia-Lymphoma; metabolism
- From: Chinese Journal of Contemporary Pediatrics 2008;10(1):14-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between human cyclin C (CCNC) gene and childhood acute lymphocytic leukemia (ALL).
METHODSThe total RNA isolated from myeloid tissues of normal children and of children with newly diagnosed ALL and from ALL cell line 6T-CEM was reversely transcribed into cDNA. Real-time fluorescence quantitative PCR method was used to detect CCNC gene expression.
RESULTSCCNC was expressed in myeloid tissues of normal children and of children with newly diagnosed ALL as well as 6T-CEM. The relative expression level of CCNC gene in children with newly diagnosed ALL was significantly lower than in normal controls (2.35 +/- 0.83 vs 13.5 +/- 0.30; P <0.05).
CONCLUSIONSCCNC gene shows lower expression in children with newly diagnosed ALL, suggesting that it may be a tumor suppressing gene in childhood ALL.
