Construction and immunogenicity evaluation of chimerical DNA vaccine of human papillomavirus type 11.
- Author:
Zhao-Hui HUANG
1
;
Li-Hua LI
;
Zhi-Jian GUO
;
Zhi-Hui LIU
;
Jin-Dong REN
;
Ming-Xu SONG
;
Xi-Ke ZHOU
;
Fei WANG
;
Zhi-Gang BI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; blood; immunology; Base Sequence; Female; Genetic Engineering; Human papillomavirus 11; genetics; immunology; Humans; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Papillomavirus Infections; blood; immunology; virology; Papillomavirus Vaccines; administration & dosage; genetics; immunology; Random Allocation; Vaccines, DNA; administration & dosage; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(3):182-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct chimerical DNA vaccine plasmid of human papillomavirus type 11 (HPV11) L1-E7, and to evaluate its immunogenicity.
METHODSMolecular cloning techniques were used to construct recombinant plasmid pcDNA3 L1-E7 as a DNA vaccine. BALB/c mice were vaccinated with DNA recombinants through muscle injection.IL-2 and gamma-INF secreted by immunized spleens lymphocyte and HPV 11 L1 or E7 specific antibodies were assayed by ELISA method. Spleens lymphocyte proliferation was measured by MTT assay.
RESULTSThe chimerical DNA plasmid of pcDNA3 L1-E7 was constructed correctly. Specific anti-HPV11 E7 and L1 antibodies, specific lymphocyte proliferation and secretions of IL-2 and gamma-INF were detected in vaccinated mice.
CONCLUSIONSpecific immune response, including cellular immunity and humoral immunity, could been detected in mice vaccinated with chimerical DNA vaccine of pcDNA3 L1-E7.
