Construction and immunogenicity evaluation of chimerical DNA vaccine of human papillomavirus type 11

VernacularTitle:人乳头瘤病毒11型L1/E7嵌合DNA疫苗的构建及其诱导的免疫效应
Author: Zhao-Hui HUANG ¹ ; Li-Hua LI ; Zi-Jian GUO ; Zhi-Hui LIU ; Jin-Dong PEN ; Ming-Xu SONG ; Xi-Ke ZHOU ; Fei WANG ; Zhi-Gang BI
Author Information

1. 苏州大学附属第四医院
Keywords: PapiUomavirus,human; Condyloma accuminata; Vaccines,DNA
From: Chinese Journal of Experimental and Clinical Virology 2009;23(3):182-184
CountryChina
Language:Chinese
Abstract: Objective To construct chimerical DNA vaccine plasmid of human papiUomavirus type 11 (HPV11) L1-E7, and to evaluate its immunogenicity. Methods Molecular cloning techniques were used to construct recombinant plasmid PeDNA3 L1-E7 as a DNA vaccine. BALB/c mice were vaccinated with DNA recombinants through muscle injection. IL-2 and γ-INF secreted by immunized spleens lymphocyte and HPV 11 LI or E7 specific antibodies were assayed by ELISA method. Spleens lymphocyte proliferation was measured by MTT assay. Results The chimerical DNA plasmid of pcDNA3 LI-E7 was constructed correctly. Specific anti-HPV11 E7 and L1 antibodies, specific lymphocyte proliferation and secretions of IL-2 and γ-INF were detected in vaccinated mice. Conclusion Specific immune response, including cellular immunity and humoral immunity, could been detected in mice vaccinated with chimerical DNA vaccine of pcDNA3 L1-E7.