Investigation epidemiology and sub-clinical infection of hepatitis E in voluntary blood donors in south Zhejiang
10.3760/cma.j.issn.1003-9279.2009.03.009
- VernacularTitle:浙南地区无偿献血者戊型肝炎亚临床感染及病毒血症情况调查
- Author:
Guo-Yong HUANG
1
;
Hai-Hong WANG
;
Xiao-Hua CHEN
;
Xiao-Mei ZHANG
;
Yan-Chai TU
;
Jian-Dao LI
;
Qiong ZHANG
;
Tong CHEN
;
Sheng-Xiang GE
Author Information
1. 浙江省温州市中心血站
- Keywords:
Blood donors;
Hepatitis E;
Infection;
Viremia
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(3):185-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the southern region of Zhejiang hepatitis E virus (HEV) infection. Methods A cluster sampling strategy was used to sample all blood donors from February to Ocwber in 2008 in Wenzhou blood center. Their blood was tested for IgG and lgM antibody against HEV. Reverse transcriptase-polymerase chain reaction(RT-PCR) and sequencing were applied to detect its genotypo and sequence homology in HEV IgM-positive specimen. Results The prevalence of anti-HEV IgG in 3044 cases of blood donors was 33.28%. IgG increased with age. There are certain increase in positive rates between the 20-year-old group and over 40 years of age group from 21.16% to 50.36%. The positive rate of IgM was 0.92% . The ratio of infection among different age group was the highest in the age range from 31to 40 years and up to 1.90%. lgG and IgM through their negative and positive analysis of samples found in their group with donors age, sex and blood type does not significantly related to each other. Nucleic acids were found in three cases through PCR amplification in all 28 cases of HEV IgM positive samples.The total positive rate was one-thousandth, of which two cases for gene 4,1 cases of infection for gene 1. Conclusion The results indicate that there was a certain percentage of HEV virus in voluntary blood donors in south Zhejiang.
