The analysis of hepatitis B virus genetic characterization from immuned children and their mother
10.3760/cma.j.issn.1003-9279.2009.03.012
- VernacularTitle:母婴阻断失败乙型肝炎病毒基因序列分析
- Author:
De-Xi CHENG
1
;
Guo-Liang YE
;
Ya LIN
;
Zhi-Hui CHEN
Author Information
1. 浙江省象山县第一人民医院
- Keywords:
Hepatitis B virus;
Disease transmission,vertical;
Mutation;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(3):194-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to investigate the characterization of mother to children transmission, the sequences of HBV were analyzed to offer the information about the effect of interrupt. Methods The sera of 75 mother with positive HBsAg are collected from 2003, and the ELISA was performed to determine the HBV infection of the child. The Large S sequence of HBV including preS and S gene are amplified and sequenced. The genotype was determined with the standard genotype sequence. The mutation ratios of group suecesssfuUy interrupted and failed compared. Results The sera of 4 pairs mother-children were HBsAg positive, including one twins. The virus genes are successful amplified. Four of HBV genotype is B and one is C. Gene of twins has mutation of T143M. 43 HBV of successfully interrupted group were sequenced. There are 37 of genotype B and 6 of genotype C. Three have the mutation in "a" dominant, and the percentage is 7%. Conclusion Most failed interrupted child have the same sequence with their mother, and the ratio is higher than the mother of successful group, however there have no statistical significance.
