Analysis of SCCmec genotyping and antimicrobial susceptibility tests in methicillin resistant Staphylococcus aureus.
- Author:
Gui-Zhen SUN
1
;
Yan-Hua YU
;
Xiu-Ying ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; pharmacology; Bacterial Proteins; genetics; China; epidemiology; Drug Resistance, Multiple, Bacterial; Genotype; Humans; Methicillin-Resistant Staphylococcus aureus; classification; drug effects; genetics; isolation & purification; Microbial Sensitivity Tests; Penicillin-Binding Proteins; Phylogeny; Staphylococcal Infections; epidemiology; microbiology
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(3):197-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the SCCmec genotyping, subtype and antimicrobial susceptibility tests in methicillin resistant staphylococcus aureus to guide the clinical treatment and provide the proof for molecular epidemiology.
METHODSTo detect mecA gene and SCCmec genetyping and subtype in 50 MRSA by PCR. According to CLSI's guideline, antimicrobial susceptibility tests were performed with disk diffusion.
RESULTSAll 50 MRSA had mecA genes. 45 strains were SCCmec III types; 3 strains were SCCmec III A types; 2 strains were SCCmec II types. There were no SCCmec I and SCCmec IV types. SCCmec II, SCCmec III and SCCmec III A type strains were all multiresistant.
CONCLUSION50 MRSA are all multiresistant. SCCmec III are the main types.
