Study on effect of enzyme linked immunosorbent assay kits of norovirus.
- Author:
Qing ZHANG
1
;
Miao JIN
;
Shu-Xian CUI
;
Na LIU
;
Lei LI
;
Zhao-Jun DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Caliciviridae Infections; diagnosis; virology; Disease Outbreaks; Enzyme-Linked Immunosorbent Assay; methods; Feces; chemistry; virology; Gastroenteritis; diagnosis; virology; Humans; Norovirus; genetics; immunology; isolation & purification; Reagent Kits, Diagnostic
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(3):227-228
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEEffects of RIDASCREEN Norovirus (C 1401) 3rd Generation kit (R-biopham AG, darmstadt, Germany) and IDEIA NLV kit (DAKOCytomation., Ely, UK) were compared for detecting human norovirus (HuNV) in fecal sample.
METHODSThe performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction (RT-PCR) by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Guang Zhou. Gene sequencing was performed to positive samples tested by RT-PCR to determine genotype compared with standard sequences.
RESULTSRT-PCR is gold standard, RIDASCREEN Norovirus (C 1401) 3rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76%.
CONCLUSIONRIDASCREEN Norovirus (C 1401) 3rd Generation kit is more Satisfactory for a preliminary screening.
