Study on effect of enzyme linked immunosorbent assay kits of norovirus

VernacularTitle:诺如病毒酶联免疫检测试剂盒效果的研究
Author: Qing ZHANG ¹ ; Miao JIN ; Shu-Xian CUI ; Na LIU ; Lei LI ; Zhao-Jun DUAN
Author Information

1. 中国疾病预防控制中心病毒病预防控制所
Keywords: Norovirus; Enzyme-Linkedimmunosorbent assay; Reverse transcriptase polymerasc chain reaction
From: Chinese Journal of Experimental and Clinical Virology 2009;23(3):227-228
CountryChina
Language:Chinese
Abstract: Objective Effects of RIDASCREEN Norovirus (C 1401) 3rd Generation kit (R-biopham AG, darmstadt, Germany)and IDEIA NLV kit (DAKOCytomation., Ely, UK)were compared for detecting human norovirus(HuNV) in fecal sample. Methods The performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction (RT-PCR) by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Gnang Zhou. Gene sequencing was performed, to positive samples tested by RT-PCR to determine genotype compared with standard sequences. Results RT-PCR is gold standard, RIDASCREEN Norovims (C 1401)3rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76%. Conclusion RIDASCREEN Norovirus(C 1401)3rd Generation kit is more Satisfactory for a preliminary screening.