Complete genome sequence analysis of Japanese encephalitis virus newly isolated In China
10.3760/cma.j.issn.1003-9279.2009.04.003
- VernacularTitle:浙江省基因Ⅰ型乙脑病毒全基因序列的测定及分析
- Author:
Rong-Hui XIE
1
;
Han-Ping ZHU
;
Shi-Hong FU
;
Yin-Kai CHENG
;
Fang XU
;
Ping-Ping YAO
;
Zhang-Nv YANG
;
Xiao-Long ZHOU
;
Zhi-Yong ZHU
Author Information
1. 浙江省疾病预防控制中心
- Keywords:
Encephalitis viruses;
Japanese;
Sequence analysis;
Phylogeny
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(4):245-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the complete genome sequence of Japanese encephalitis virus(JEV) strain X J69 isolated in ZheJiang province and explore its evolution. Methods Overlapping primers were designed according to the fuU-length genomes from C, enBank. RT-PCR was used to amplify the fragments and RT-PCR products were cloned T vector, sequenced and analyzed. Results The genome of strain XJ69 and XJP613 were 10 964 nucleotides in length with a single open reading frame encoding 3432 amino acids. Comparison of the complete geoome sequences of different JEV isolates showed XJ69 and XJP613 were 83.5%-99.2% and 83.4%-99.4% nucleotide sequence homology among them respectively, which resu]ted in 94.8%-99.7% amino acid sequence homology. Phylogetic analysis through PrM/C ,E and full-length genome showed that the X J69 and XJP613 strain belonged to ganotype Ⅰ. Conclusion The nucleoiede sequence and deduced amino acid sequence of X J69 and XJP613 strain were similar to that of those of genotype Ⅰ of Japanese encephalitis virus. It belonged to genotype Ⅰ and were close to the isolates SH17M-07.
