Molecular characterization of Japanese Encephalitis Virus isolated from Gansu province in 2008
10.3760/cma.j.issn.1003-9279.2009.04.005
- VernacularTitle:2008年甘肃省新分离乙脑病毒的分子生物学特征
- Author:
Ming-Hua LI
1
;
Shi-Hong FU
;
Yun FENG
;
Xiao-Yan GAO
;
You-Gang ZHAI
;
De-Shan YU
;
Guo-Tai LI
;
Yu-Xin JIA
;
Guo-Dong LIANG
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Encephalitis viruses;
Japanese;
Genotype;
Sequence analysis;
Phylogeny
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(4):251-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene. Methods Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4. Results The six JEV strains were clustered in genotype Ⅰ. 87.5%-87.9% identity in nucleotide sequence and 96.8%--97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain. Conclusion Genotype Ⅰ JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.
