Frequency, distribution of CD4+, CD8+ T cells and expression of CD38 in gastric mucosa of HIV Infections
10.3760/cma.j.issn.1003-9279.2009.04.008
- VernacularTitle:HIV感染后胃黏膜CD4+、CD8+T细胞数量分布及CD38表达的改变
- Author:
Lei HUANG
1
;
Xian-Zhi ZHOU
;
Jing-Min ZHAO
;
Hao WU
;
Rui JIN
;
Xin MENG
;
Deng PAN
;
Shi-Jie ZHANG
Author Information
1. 解放军第302医院
- Keywords:
HIV;
Acquired immunodeficiency syndrome;
Gastric mucosa
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(4):261-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the frequencies and distribution of CD4+ T ceils and CD8+ T cells as well as the changes of immune activation in gastric mucesa of HIV-infected individuals. Methods 42 HIV-infected individuals were recruited into this investigation, and 36 patients had definite diagnosis of clinical stage.Biopsy of gastric mucosal tissues was performed by fiberoptic gastrescope including 10 normal people as a control group. Then, immunohistochemistry was used to detect expression of CD4, CD8 and CD38 in gastric mucosa, and the distinctions among three groups were analyzed with LEICA Qwin image analysis system. Results (1) Compared with asymptomatic HIV carriers and control group, CD4+ T cells remarkably decreased in the gastric mucosa of AIDS patients (P < 0.01). In gastric mucosa of asymptomatic HIV carriers, there were still some CD4+ T cells in lymphoid follicles and stroma where CD4+ T cells were unevenly distributed, the frequency of CD4+ T cells was not significandy different between asymptomatic HIV carriers and control group (P > 0.05) ; (2) Phenomenon of CD8+ T cells infiltrating mucosal epithelium and gland was general in HIV-infected individuals. CD8+ T cells took on local excessive hyperplasia in gastric mucosa of some individuals. As compared with control group, CD8+ T cells markedly increased in gastric mucosa of infected individuals (P < 0.01), but the distinction of asymptomatic HIV carriers and AIDS patients was not significant (P > 0.05); (3) CD38-expressing cells mainly distributed over gastric mucosai surface to superficial layer(1/3-2/3 layer) of HIV-infected individuals, and was more intensive than control group (P≤ 0.01), but there was not noticeable difference between asymptomatic HIV carriers and AIDS patients (P > 0.05). Conclusion The frequencies and distribution of gastric mucosal CD4+ T cells of HIV-infected individuals were closely correlated with progression of disease. Disfunction of mucosal immune system which was resulted from HIV infection and injury of CD4+ T cells could be an important cause of CD8+ T cells increasing and CD38-expressing enhancement.
