Expression and signiflcance of liver X receptor-α in nonalcoholic fatty liver disease in rats
10.3760/cma.j.issn.1003-9279.2009.04.009
- VernacularTitle:LXR-α在大鼠非酒精性脂肪肝组织中的表达及其意义
- Author:
Jie CHEN
1
;
Rui-Dan ZHENG
;
Chen-Run XU
;
Wei-Ping WU
Author Information
1. 解放军第175医院
- Keywords:
Fatty liver;
Liver X receptor-α;
Genes;
Rats;
Sprague dawley
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(4):265-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective Preparing rat model of non-alcoholic fatty liver disease by fat-rich diet to observe the expression and the role of LXR-α in rat nonalcoholic fatty liver disease. Methods Thirty-six SD rats were randonmized into basic diet-control group and high-fat diet group. Each of the two groups was subdivided into 3 subgroups(4,8,12 weeks). Changes in animal weight, liver exponent and the level of TG and TC in serum and liver were observed dynamically. Meanwhile, the expression of hepatocyte LXR-α and SREBP-1c were assayed by Reverse transcript-polymerase chain reaction at 4,8, 12 weeks. The level of steatosis was observed under light microscope after haematoxylon-eosin (HE)staening. Results Compared with control group, body weight, liver exponcnt,TG and Tc in serum and liver were increased dynamically in model groups. Compared with control group, the mRNA of LXR-α and SREBP-1c were obviously increased dynamically in model groups(P < 0.05). Conclusion The increase of LXR-α and SREBP-1c in liver may be eoneered with energy disorder and closely associated with the activity of inflammation and the severity of the liver damage in NAFLD rats.
