Research of ITK regulation on mouse spleen lymphocytes proliferation and differentiation.
- Author:
Zong-Hui XIAO
;
Feng HE
;
Hai-Lan YAO
;
Ji-Sheng HAN
;
Zhe-Wei LIU
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cell Proliferation; Cytokines; genetics; immunology; Lymphocytes; cytology; immunology; Male; Mice; Mice, Inbred BALB C; Protein-Tyrosine Kinases; genetics; immunology; Spleen; cytology; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(4):269-271
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEBy using the RNAi method to inhibit Itk protein expression specificity, to observe lymphocytes proliferation and cytokines production, verify its function as a drug target.
METHODSDesigned siRNA aims at Itk sequence according to its sequence and solid structure, then electrotransfected into mouse spleen lymphocytes, We validated the decrease of Itk protein by Western-Blot, and detected the change of the cell proliferation by MTS and the change of inflammatory cytokines by ELISA.
RESULTSItk protein can be suppressed by Itk-siRNA, there were significantly reduced compared to its control group on cell proliferation as well as cytokine secretion such as IL-2, IL-4, IL-5, IFN-gamma. They all have statistical difference (P < 0.05).
CONCLUSIONItk has an important immunomodulatory effect in mouse spleen lymphocytes proliferation and secretion of inflammatory cytokines.This can supply an experimental basis to regard Itk as drug target for inflammation therapy.