Research of ITK regulation on mouse spleen lymphocytes proliferation and differentiation

VernacularTitle:ITK蛋白调节小鼠脾淋巴细胞增殖分化的研究
Author: Zong-Hui XIAO ¹ ; Feng HE ; Hai-Lan YAO ; Ji-Sheng HAN ; Zhe-Wei LIU
Author Information

1. 首都儿科研究所
Keywords: Protein-Tyrosine kinase; RNA; Cells; cultured; Cytokines
From: Chinese Journal of Experimental and Clinical Virology 2009;23(4):269-271
CountryChina
Language:Chinese
Abstract: Objective By using the RNAi method to inhibit Itk protein expression specificity,to observe lymphocytes proliferation and cytokines production, verify its function as a drug target. Methods Designed siRNA aims at Itk sequence according to its sequence and solid structure, then electrotransfected into mouse spleen lymphocytes, We validated the decrease of Itk protein by Western-Blot, and detected the change of the cell proliferation by MTS and the change of inflammatory cytokines by ELISA. Results Itk protein can be suppressed by Itk-siRNA, there were significantly reduced compared to its control group on cell proliferation as well as cytokine secretion such as IL-2, IL-4, IL-5, IFN-T. They all have statistical differenc (P < O. 05). Conclusion Itk has animportant immunomodulatory effect in mouse spleen lymphocytes proliferation and secretion of inflammatory cytokines. This can supply an experimental basis to regard Irk as drug target for inflammation therapy.