Epitope screening of influenza A (H3N2) by using phage display library.

Author: Yan-Wei ZHONG ¹ ; Dong-Ping XU ; Xiao-Dong LI ; Jiw-Zeng DAI ; Biao XU ; Le LI
Author Information

1. Viral hepatitis research laboratary, Institute of Infectious Disease of P1A 302 Hospital, Beijing 100039, China.
Publication Type:Journal Article
MeSH: Epitope Mapping; Humans; Influenza A Virus, H3N2 Subtype; chemistry; genetics; immunology; Peptide Library
From: Chinese Journal of Experimental and Clinical Virology 2009;23(4):272-274
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo screen the influenza A (H3N2) mimotopes by using phage display library.
METHODSUsing influenza A (H3N2) monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
RESULTS21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2).
CONCLUSIONInfluenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza virals vaccine development.