Epitope screening of influenza A (H3N2) by using phage display library

VernacularTitle:应用噬菌体表面展示技术筛选流感病毒H3N2抗原模拟表位
Author: Yan-Wei ZHONG ¹ ; Dong-Ping XU ; Xiao-Dong LI ; Jiu-Zeng DAI ; Biao XU ; Le LI
Author Information

1. 解放军第302医院
Keywords: Orthomyxoviridae; Epitapes; Peptide library
From: Chinese Journal of Experimental and Clinical Virology 2009;23(4):272-274
CountryChina
Language:Chinese
Abstract: Objective To screen the influenza A (H3N2) mimotopes by using phage display library. Methods Using influenza A (H3N2) monaclonai antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. Results 21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2). Conclusion Influenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza vitals vaccine development.