Construction and application of a genechip method for detection of hepatitis B virus lamivudine-resistant mutants and basal core promotor/Pre-C mutants.
- Author:
Bing LI
1
;
Bo-Ping ZHOU
;
Jin-Fu PENG
;
Li-Yan CHEN
;
Wen ZHANG
;
Wei TANG
;
Zhao-Qin WANG
;
Zhong-Hua YIN
;
Liu-Mei XU
;
Rui-Ling LUO
;
Xiao-He LI
;
Sai-Yun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Antiviral Agents; pharmacology; Base Sequence; Drug Resistance, Viral; Hepatitis B; drug therapy; virology; Hepatitis B Core Antigens; genetics; Hepatitis B virus; drug effects; genetics; isolation & purification; Humans; Lamivudine; pharmacology; Molecular Sequence Data; Mutation; Oligonucleotide Array Sequence Analysis; methods; Promoter Regions, Genetic
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(4):309-312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe objective of this research is to construct a clinic-usable genechip method for detection of hepatitis B virus lamivudine-resistant mutants and basal core promotor/Pre-C mutants, compare this method with DNA sequencing to investigate this genechip's character (sensity, specificity, stability and practicability in clinic) and apply it in clinic.
METHODSThis genechip detection method can detect the DNA and 8 mutative site of HBV, include 3 lamivudine-resistant mutation site(No. 180, 204, 207 site in DNA polymerase gene), 5 HBeAg escape-related mutation site (nt 1896, 1899, 1862, 1764,1762 site in BCP/Pre-C region).The results of genechip method was verified by DNA sequencing.
RESULTSIn detecting HBV DNA, the results of genechip were agree with 100% of the results of DNA sequencing. In detecting HBV mutants, 251 sites (in 32 samples, 256 sites) showed the same results using both methods, and only 5 sites were not completely match (P > 0.05). In these 5 sites, genechip methods got multi-infection results, but sequencing got single-infection results.
CONCLUSIONThese results suggest that genechip method has the same positive rate and almost these same specificity with DNA sequencing method, and is better than DNA sequencing method in detecting multi-infected HBV strains. [Key words]
