Construction and application of a genechip method for detection of hepatitis B virus lamivudine-resistant mutants and basal core promotor/Pre-C mutants
10.3760/cma.j.issn.1003-9279.2009.04.023
- VernacularTitle:HBV拉米夫定耐药株及BCP、Pre-C突变株芯片检测方法的应用研究
- Author:
Bing LI
1
;
Bo-Ping ZHOU
;
Jin-Fu PENG
;
Li-Yan CHEN
;
Wen ZHANG
;
Wei TANG
;
Zhao-Qin WANG
;
Zhong-Hua YIN
;
Liu-Mei XU
;
Rui-Ling LUO
;
Xiao-He LI
;
Sai-Yun LIU
Author Information
1. 广东医学院附属深圳第三人民医院
- Keywords:
Oligonucleotide probes;
Hepatisis B Virus;
Sequence Analysis;
DNA;
Mutation
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(4):309-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this research is to construct a clinic-usable genechip method for detection of hepatitis B virus lamivudine-resistant mutants and basal core promotor/Pre-C mutants, compare this method with DNA sequencing to investigate this genechip's character (semity, specificity, stability and practicability in clinic) and apply it in clinic. Methods This genechip detection method can detect the DNA and 8 mutative site of HBV, include 3 lamivudine-resistant mutation site (No. 180, 204, 207 site in DNA polymerase gene) ,5 HBeAg escape-related mutation site(nt 1896, 1899, 1862, 1764, 1762 site in BCP/Pre-C region).The results of genechip method was verified by DNA sequncing. Results In detecting HBV DNA, the results of genechip were agree with 100% of the results of DNA sequencing. In detecting HBV mutants, 251 sites (in 32 samples, 256 sites) showed the same results using both methods, and only 5 sites were not completely match(P >0.05). In these 5 sites, genechip methods got multi-infection results, but sequencing got single-infection results.Conclusion These results suggest that genechip method has the same positive rate and almost these same specificity with DNA sequencing method, and is better than DNA sequencing method in detecting multi-infected HBV strains.
