Construction and expression of hepatitis B virus envelope protein combined with core protein with two multiple cloning sites vector

VernacularTitle:乙型肝炎病毒外膜蛋白和核壳蛋白双表达质粒的构建和表达
Author: Hong-Hui SHEN ¹ ; Su-Jun LIU ; Lei GUO ; Jun HOU ; Zhi-Jie WANG ; Shao-Jie XIN ; Bing-Ke BAI ; Yan HU ; Xiang-Hui YU ; Wei KONG ; Pan-Yong MAO
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1. 解放军第302医院
Keywords: Hepatitis B virus; Expressed sequence tags; Viral envelope protcins; Nucleoproteins
From: Chinese Journal of Experimental and Clinical Virology 2009;23(4):313-315
CountryChina
Language:Chinese
Abstract: Objective To develop a eoexpression plasmid which expressing envelope protein and nucleoprotein of hepatitis B virus and know of its expressing efficiency. Methods The plasmid coexpressing envelope protein and nucleoprotein of hepatitis B virus under the CMV promoter respectively was constructed by gene recombination. Cellular expression was assessed by ELISA. Results Multiple cloning site was inserted into expression vector contain hepatitis B virus PreS2-S gene. And expression unit containing hepatitis B virus PreC-C was cloned into it. HBsAg and HBeAg was detected both in the culture supernatant and in the cells. Conclusion The coexpressing plasmid was constructed successfully and it can express effectively in vitro. This has provided a basis for further research of the therapeutic HBV DNA vaccine.