Clinical research of HPV DNA detection and HPV RNA detection in single time.
- Author:
Wen-bo LU
1
;
Zhi-nan JIANG
;
Shun-mei CHEN
;
Shi-rong QIN
;
Lin-jun ZHAO
;
Hai-tao YANG
;
Dan-hua SHI
;
Xue-mei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alphapapillomavirus; genetics; isolation & purification; DNA, Viral; genetics; Female; Genetic Techniques; Humans; Middle Aged; Oncogene Proteins, Viral; genetics; Papillomavirus Infections; virology; RNA, Viral; genetics; Vaginal Smears; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(5):378-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo research the application of the single time detection of HPV E6/E7 mRNA and HC2-HPV-DNA in cervical screening project.
METHODSWe detected both HPV E6/E7 mRNA and HC2-HPV-DNA of each sample which collected from 130 cervical disease patients' cervix during Jan. 2008 and July. 2009. TCT results were taken as standard to evaluate the diagnostic accuracy of the above two test methods in detecting high-grade cervical disease.
RESULTS82.3% (107/130)women were confirmed to infect HPV by HC2-HPV-DNA detection, and 40.0% (52/130) women were confirmed to infect HPV by HPV E6/E7 mRNA detection, there was no significant difference between the two results (chi2 = 24.5, P < 0.05). The sensitivity, specificity, positive predictive value, negative predictive value of HC2-HPV-DNA detection were 90.1%, 22.1%, 37.4% and 82.6%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value of HPV E6/E7 mRNA detection were 65.9%, 73.3%, 55.8% and 80.8%, respectively.
CONCLUSIONIn clinical cervical screening project of single time, the combination of HC2-HPV-DNA detection and HPV E6/E7 mRNA detection wick take on more potential value than applying each of them alone. RNA;
