Development a method of quantitative assay for enterovirus 71.
- Author:
Jing XU
1
;
Wen-yu CUI
;
Shu-xiang LI
;
Jing LIU
;
Xin-yi WANG
;
Lei CHEN
;
Xin-Liang SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Viral; analysis; immunology; Cell Line; Enterovirus; immunology; isolation & purification; Enterovirus Infections; diagnosis; immunology; virology; Enzyme-Linked Immunosorbent Assay; methods; Humans; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(5):388-390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative assay for enterovirus 71, this can be used in detecting the virus content during vaccine development and production.
METHODSWe established the method of quantitative assay for EV71 by using double antibody sandwich ELISA. The sensitivity, accuracy,precision and specificity of the method were evaluated.
RESULTSWe developed an ELISA method to quantitative assay for EV71. The quantitation limit of the method is 0.23 microg/ml and the quantitation scope of the method is 7.32-0.23 microg/ml, the coefficient correlation is R2 = 0.9976; The method showed good accuracy, precision and specificity. The recovery is between 90%-110% and the variation coefficient is lower than 10%.
CONCLUSIONAn ELISA method was developed for the quantitative assay of EV71 virus, which can be used for the rapid quantitative determination of EV71 virus during vaccine development and production.
