OBJECTIVEBuilding a method which can examines virus pathogenic in gastroenteritis excrement specimen.

METHODSChoosing six positive specimens which tested in our laboratory, include adenovirus, calicivirus, rotavirus, bocavirus, astrovirus and enterovirus. Through sequence-independent single primer amplification(SISPA) constructs a gene bank. Looks up the viral gene fragment in gene bank.

RESULTSObtaining corresponding viral acid sequence in six specimens.

CONCLUSIONThis research can examine enterovirus and the virus which cause diarrhea, It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present.