Characterization of pseudotyped viruses coated with hemagglutinin of H5N1 avian influenza
10.3760/cma.j.issn.1003-9279.2009.06.001
- VernacularTitle:H5N1禽流感病毒HA假病毒的构建及特性研究
- Author:
Yu-Song ZHANG
1
;
Da-Yan WANG
;
Rong-Bao GAO
;
Jie DONG
;
Zi LI
;
Ye ZHANG
;
Tian BAI
;
Min WANG
;
Le-Ying WEN
;
Yue-Long SHU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Viroids;
Influenza A virus;
avian;
Hemagglutinins
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(6):401-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct pseudovirus beating H5N1 HA based on a lentivirus vector system.Then we study the biological feature of the pseudovirus.With the newly established viral particles,we performed the serological tests.Methods H5N1 avian influenza vires that isolated from human case was cloned to construct pLP-HA,then pLP-HA co-transfected with lentivirus vector plasmids pLP1,pLP2 and pEmGFP into 293T cells.The supematant was harvested 48h post-transfection.Concentrated by super centrifuge,the pseudotyped viruses were analyzed by infection test,HA test and micro-neutralization test.At the same time,optimized HA gene and a Vietnam H5N1 HA gene were used to construct pseudotyped virus for comparison.Results Pseudotyped virus particles can be observed with electronic microscope.Western-blot revealed that HA glycoprotein can be expressed in the virions. Our neutralization assay by using the pseudovimses was comparable with the conventional microneutralization assay with wild-type viruses.A hish degree of correlation was detected.Conclusion Pseudotyped Viruses coated with HA of H5N1 High Pathogenic Avian lnfluenza were successfully constructed;it can be used to for the microneutralization assay.The HA gene from different sources affect the efficiency of the packaging of the pseudovirus.But the optimized HA gene can not obviously improve packaging efficiency of the pseudovims.
